CAT、POD和APX 3种酶活力SI单位的换算

doi:10.13998/j.cnki.issn1002-1248.2015.08.039

摘要/Abstract

摘要： 目前CAT、POD和APX等氧化还原反应酶类的酶活单位在中文期刊中存在着极大的差别,并且在很多期刊中,这3种酶所使用的单位也不是国际单位制（SI）单位,为了进一步推广SI单位的使用,从springer数据库中下载了发表于2013年—2014年含有这3种酶的45篇文章, CAT、POD和APX的活性单位可以分别通过3个公式将原来使用U作为酶活单位的方式换算为以kat作为其活性单位的表示,其中CAT通过H₂O₂在240 nm下的43.6 L/(mol·cm)摩尔吸收系数转换为SI单位（以kat表示）,POD的可以通过470 nm下愈创木酚氧化产物的26.6 L/(mmol·cm)摩尔吸收系数由U表示的单位换算为以kat的SI单位,APX的活性单位可以通过反应物抗坏血酸在290 nm下的2.8 L/(mmol·cm)换算为SI单位。

关键词: 过氧化氢酶, 过氧化物酶, 抗坏血酸过氧化物酶, SI单位, 摩尔吸收系数

Abstract: In order to spread the SI system and change the chaotic situation about the enzyme activity unit of CAT and POD and APX, we downloaded 45 papers published in 2013 and 2014 containing CAT, POD and APX from springer database, and analyzed the catalytic mechanism and the method for determination of enzyme activity and the definition method of enzyme activity about CAT, POD and APX. We found that these enzyme activities of three enzymes were expressed by reduction rate of reactant amount or increasing rate of resultant amount which they catalyzed, and the amount which catalyzed could be detected at a certain wavelength. So through molar extinction coefficient of the reactant or resultant at its certain wavelength, all kinds of enzyme activity unit of CAT and POD and APX could be convert to IS unit by using three formulas, for example, extinction coefficient for hydrogen peroxide was 43.6 L/ (mol·cm) at 240 nm, for tetraguaiacol was 26.6 L/ (mmol·cm) at 470 nm, extinction coefficient for ascorbic acid was 2.8 L/ (mmol·cm) at 290 nm.

Key words: CAT

中图分类号: G250

刘昌来, 田亚玲, 王国栋, 郑琰燚, 李燕文. CAT、POD和APX 3种酶活力SI单位的换算[J]. 农业图书情报学刊, 2015, 27(8): 160-163.

LIU Chang-lai, TIAN Ya-ling, WANG Guo-dong, ZHENG Yan-yi, LI Yan-wen. Conversion of Enzyme Activity unit (non-SI unit) to SI unit for CAT, POD and APX[J]. Journal of library and information science in agriculture, 2015, 27(8): 160-163.

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