CAT、POD和APX 3种酶活力SI单位的换算

doi:10.13998/j.cnki.issn1002-1248.2015.08.039

摘要/Abstract

摘要： 目前CAT、POD和APX等氧化还原反应酶类的酶活单位在中文期刊中存在着极大的差别,并且在很多期刊中,这3种酶所使用的单位也不是国际单位制（SI）单位,为了进一步推广SI单位的使用,从springer数据库中下载了发表于2013年—2014年含有这3种酶的45篇文章, CAT、POD和APX的活性单位可以分别通过3个公式将原来使用U作为酶活单位的方式换算为以kat作为其活性单位的表示,其中CAT通过H₂O₂在240 nm下的43.6 L/(mol·cm)摩尔吸收系数转换为SI单位（以kat表示）,POD的可以通过470 nm下愈创木酚氧化产物的26.6 L/(mmol·cm)摩尔吸收系数由U表示的单位换算为以kat的SI单位,APX的活性单位可以通过反应物抗坏血酸在290 nm下的2.8 L/(mmol·cm)换算为SI单位。

关键词: 过氧化氢酶, 过氧化物酶, 抗坏血酸过氧化物酶, SI单位, 摩尔吸收系数

Abstract: In order to spread the SI system and change the chaotic situation about the enzyme activity unit of CAT and POD and APX, we downloaded 45 papers published in 2013 and 2014 containing CAT, POD and APX from springer database, and analyzed the catalytic mechanism and the method for determination of enzyme activity and the definition method of enzyme activity about CAT, POD and APX. We found that these enzyme activities of three enzymes were expressed by reduction rate of reactant amount or increasing rate of resultant amount which they catalyzed, and the amount which catalyzed could be detected at a certain wavelength. So through molar extinction coefficient of the reactant or resultant at its certain wavelength, all kinds of enzyme activity unit of CAT and POD and APX could be convert to IS unit by using three formulas, for example, extinction coefficient for hydrogen peroxide was 43.6 L/ (mol·cm) at 240 nm, for tetraguaiacol was 26.6 L/ (mmol·cm) at 470 nm, extinction coefficient for ascorbic acid was 2.8 L/ (mmol·cm) at 290 nm.

Key words: CAT

中图分类号:

G250

刘昌来, 田亚玲, 王国栋, 郑琰燚, 李燕文. CAT、POD和APX 3种酶活力SI单位的换算[J]. 农业图书情报学刊, 2015, 27(8): 160-163.

LIU Chang-lai, TIAN Ya-ling, WANG Guo-dong, ZHENG Yan-yi, LI Yan-wen. Conversion of Enzyme Activity unit (non-SI unit) to SI unit for CAT, POD and APX[J]. , 2015, 27(8): 160-163.

参考文献

[1] Murshed R,Lopez-Lauri F,Sallanon H. Effect of water stress on antioxidant systems and oxidative parameters in fruits of tomato (Solanum lycopersicon L, cv. Micro-tom)[J]. Physiology and Molecular Biology of Plants, 2013,19(3):363-378.
[2] Yildiztugay E, Ozfidan-Konakci C, Kucukoduk M. Modulation of osmotic adjustment and antioxidant status in salt-stressed leaves of Thermopsis turcica[J]. Acta Physiologiae Plantarum, 2014,36(1):125-138.
[3] Pandey N. Antioxidant responses and water status in Brassica seedlings subjected to boron stress[J]. Acta Physiologiae Plantarum,2013,35(3):697-706.
[4] 王丽芳,吴克力,郭学兰,等.几种自定义酶活性单位常见错误辨析及规范使用建议[J].中国科技期刊研究,2013,24(5):1009-1011.
[5] Nomenclature E. Recommendations 1964 of the International Union of Biochemistry[J]. An~ sterdam: Elsevier Publication Comp,1965.
[6] International Union of Biochemistry. Commission on Enzymes. Comprehensive Biochemistry: Report of the Commission on Enzymes of the International Union of Biochemistry[M]. Elsevier Publishing,1964.
[7] 全国量和单位标准化技术委员会.GB 3100-1993 国际单位制及其应用[S].北京:中国标准出版社,1993.
[8] Commission on Biochemical Nomenclature, Nomenclature Committee International Union of Biochemistry. Enzyme nomenclature: Recommendations(1972)of the Commission on Biochemical Nomenclature on the nomenclature and classification of enzymes together with their units and the symbols of enzyme kinetics[M]. Elsevier,1973.
[9] Barry N. Taylor,Physics Laboratory. The International System of Units (Si)[M]. 2008 Edition. Gaithersburg:Supersedes NIST Special Publication,2008.
[10] De Simone V, Soccio M, Borrelli G M, et al. Stay-green trait-antioxidant status interrelationship in durum wheat (Triticum durum) flag leaf during post-flowering[J]. Journal of plant research,2014,127(1):159-171.
[11] Wu H F, Liu X L,Zhao J M,et al. Regulation of metabolites, gene expression, and antioxidant enzymes to environmentally relevant lead and zinc in the halophyte Suaeda salsa[J]. Journal of Plant Growth Regulation,2013,32(2):353-361.
[12] Ramirez-Higuera A, Quevedo-Corona L, Paniagua-Castro N, et al. Antioxidant enzymes gene expression and antihypertensive effects of seaweeds Ulva linza and Lessonia trabeculata in rats fed a high-fat and high-sucrose diet[J]. Journal of Applied Phycology, 2014, 26(1): 597-605.
[13] Aebi H.Catalase in vitro [J]. Meth Enzymol.1984,(105):121-126.
[14] Chance B, Maehly A C. Assay of catalases and peroxidases[J]. Methods in enzymology, 1955,(2): 764-775.
[15] Van Gelder B F, Slater E C. The extinction coefficient of cytochrome c[J].Biochimica et biophysica acta,1962,58(3):593-595.
[16] Subhashini Devi P, Satyanarayana B, Arundhati A, et al. Activity of antioxidant enzymes and secondary metabolites during in vitro regeneration of Sterculia urens[J] Biologia Plantarum , 2013,57(4):778-782.
[17] Diao M, Ma L, Wang J W, et al. Selenium promotes the growth and photosynthesis of tomato seedlings under salt stress by enhancing chloroplast antioxidant defense system[J]. Journal of Plant Growth Regulation,2014,33(3):671-682.