Abstract: Through the development and application of hypermedia e-book reading system, this research provided a reference case study and an effective application mode. It analyzed the current situation and development trend of e-book, and explored the information organization model and the application field of hypermedia e-books, and raised propose of establishing a hypermedia e-book system, taking Jinan University Library as an example to discuss how to develop and implement the system. The results showed that the system could help to promote the resource display and service of library, and impulse the hypermedia e-books reading as well as the research of hypermedia e-book.