Abstract: The ancient books are the carrier of human knowledge and culture. Under the theoretical guidance and technical support of digital humanities, digital processing, storage, organization and analysis of ancient books can not only realize the regenerative protection of ancient books, but also realize the dissemination and inheritance of the cultural knowledge carried by the ancient books. By studying the practice of ancient books digitization, and the theory and technology of digital humanities, this paper discussed the application of digital humanistic theory and technology in the development and utilization of ancient books, so as to realize the efficient use of ancient books.