中文    English

›› 2016, Vol. 28 ›› Issue (10): 21-25.doi: 10.13998/j.cnki.issn1002-1248.2016.10.004

• Resources construction • Previous Articles     Next Articles

Detection on Rice Wx and fgr Gene Distribution in Heilongjiang Rice Germplasm Resource by HRM Functional Markers

GUO Zhen-hua1,2, LUO Wen-long2, HUANG Cui-hong2, CHEN Zhi-qiang2, HUANG Xiao-qun1, LIU Chuan-xue1, ZHANG Lan-min1   

  1. 1. Jiamusi Rice Research Institute of Heilongjiang Academy of Agricultural Sciences, Jiamusi 154026;
    2. National Engineering Research Centre of Plant Space Breeding, Guangzhou 510642, China
  • Received:2016-03-22 Online:2016-10-05 Published:2016-09-29

Abstract: As the most important quality characters of rice grain, amylose content and fragrance are always hot issues in scientific research on rice. HRM (High Resolution Melting) technology as a new PCR analysis technology in recent years, with the characters of simple, fast and high resolution, is widely concerned by the broad mass of researchers. In order to determine the distribution of Wx and fgr gene in germplasm resource of Heilongjiang province, 67 main cultivated varieties and germplasm resource in Heilongjiang province were genotyped by the two HRM(high resolution melting)-based functional markers Wx-a/b and fgr-E7FNP. The results showed that the homozygous gene Wxb existed in all the 67 germplasm resource; 4 germplasm resource contained the homozygous gene fgr, namely Ha99352, Longjingxiang1, Zhonglongxiangjing1 and Longjiao103970, and the rest contained the homozygous gene BAD2.

Key words: Rice

CLC Number: 

  • S331
[1] Wang Z Y, Wu Z L, Xing Y Y, et al.Nucleotide sequence of rice waxy gene[J].Nucl Acids Res,1990,18(19):5898.
[2] Cai X L, Wang Z Y, Xing Y Y, et al.Aberrant splicing of intron 1 leads to the heterogeneous 5′UTR and decreased expression of waxy gene in rice cultivars of intermediate amylose content[J].Plant J,1998,14(4):459-465.
[3] Sano Y.Differential regulation of waxy gene expression in rice endosperm [J].Theor.Appl.Genet.,1984,(68):467-473.
[4] 蔡秀玲,刘巧泉,汤述翥,等.用于筛选直链淀粉含量为中等的籼稻品种的分子标记[J].植物生理与分子生物学学报,2002,28(2):137-144.
[5] 罗文龙,郭涛,周丹华,陈海英,王慧,陈志强等.利用基于HRM的功能标记分析水稻Wx和fgr的基因型[J].湖南农业大学学报(自然科学版),2013,39(6):1 007-1 032.
[6] Buttery R G, Ling L C, Juliano B O, et al.Cooked rice aroma and 2-acetyl-1-pyrroline[J].Agric Food Chem,1983,(31):823-826.
[7] Aliss.Inheritance studies for aroma in two aromatic varieties of Pakistan [J].IRRN, 1993,(18):65.
[8] Bradbury L M T, Fitzgerald T L, Henry R J, et al.The gene for fragrance in rice [J].Plant Biotechnol J,2005,(3):363-370.
[9] Chen S H,Yang Y,Shi W W,et al.Badh2,Encoding betainealdehyde dehydrogenase,inhibits the biosynthesis of 2-acetyl-1-pyrroline,a major component in rice fragrance [J].Plant Cell, 2008, 20(7): 1 850-1 861.
[10] Vossen R, Aten E, Roos A, et al..High-Resolution melting analysis (HRMA)-More than just sequence variant screening [J].Human Mutation, 2009, 30(6): 860-866.
[11] De Koeyer D, Douglass K, Murphy A, et al..Application of high-resolution DNA melting for genotyping and variant scanning of diploid and autotetraploid potato [J].Molecular Breeding,2010,25(1):67-90.
[12] Li Y D, Chu Z Z, Liu X G, et al..A cost-effective high-resolution melting approach using the EvaGreen dye for DNA polymorphism detecting and genotyping in plants [J].Journal of Intergrative Plant Biology, 2010, 52(12): 1 036-1 042.
[13] Hofinger B J, Jing H C, Hammond-Kosack K E, et al..High-resolution melting analysis of cDNA-derived PCR amplicons for rapid and cost-effective identification of novel alleles in barley [J].Theor.Appl.Genet., 2009,119(5):851-865.
[14] 赵均良,张少红,刘斌.应用高分辨率熔解曲线技术分析水稻分子标记基因型[J].中国农业科学,2011,44(18):3701-3708.
[15] McCouch S R, Kochert G, Yu Z H, Khush G S, Coffman W R, Tanksley S D.Molecular mapping of rice chromosomes.Theor.Appl.Genet., 1988,(76):815-820.
[16] 余四斌.水稻重要性状的等位基因研究进展[J].分子植物育种,2010,8(6):1059-1067.
[17] Bligh, HFJ., Hill, RL., Jones, C.A.A microsatellite sequence closely linked to the waxy gene of Ores sativa[J].Euphytica, 1995,(86):83-85.
[18] Rakszegi M, Kisgyorgy B N, Tearall K, et al..Diversity of agronomic and morphological traits in a mutant population of bread wheat studied in the Healthgrain program [J].Euphytica, 2010,174(3):409-421.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!