中国土壤微生物领域研究现状与前沿热点分析

doi:10.13998/j.cnki.issn1002-1248.21-0054

摘要/Abstract

摘要： [目的/意义] 土壤微生物是土壤最活跃的组成,关乎土壤生态系统的健康。因此摸清土壤微生物研究领域的热点、前沿及趋势,有助于科研人员把握选题内容及方向。[方法/过程] 以CNKI中国学术期刊收录的2010年至2019年土壤微生物的核心期刊文献为分析对象,应用CiteSpace V软件分析发文作者、机构及期刊的分布特点,并绘制关键词共现、突现词分布及关键词突现术语时线图的知识图谱。[结果/结论] 每年中国土壤微生物相关文献量均达到400篇以上,该领域研究持续保持较高热度。以西北农林科技大学和中国科学院大学为核心的重点研究单位,以沈其荣、杨殿林、毛志泉为核心的三大作者群,为该领域发展做出了突出贡献。研究热点随时间推进有所演变,统分为四大模块：第一,间接反映土壤微生物的指标（土壤酶活性,土壤呼吸,土壤微生物量碳、氮）;第二,研究方法的成熟发展（磷脂脂肪酸、变性梯度凝胶电泳、Biolog-ECO技术、高通量测序）;第三,土壤微生物的直观表征（微生物群落结构及功能多样性）;第四,影响因素的探究（土壤类型及土地利用方式、施肥、农作物、氮沉降、生物炭）。高通量测序等分子生物学方法及系统生物学方法的应用、生物炭开发利用和氮沉降影响及改良措施研究作为前沿应被持续关注和深入探讨。

关键词: 土壤微生物, CiteSpace, 研究热点, 前沿趋势

Abstract: [Purpose/Significance] Soil microorganism is the most active component of soil, which is the key factor of a healthy soil ecosystem Therefore, to investigate the hotspots, frontiers and directions of soil microorganism research is helpful for researchers to understand research topics and directions. [Method/Process] Using CiteSpace V software papers of soil microbiology published by key journals from 2010 to 2019 collected by CNKI were used to analyze the distribution characteristics of authors, institutions and journals, and to draw the knowledge graph of keyword co-occurrence, burst terms distribution and the time line map of keyword burst terms. [Results/Conclusions] There are more than 400 articles about soil microorganism area published in China every year, and the research in this field continues to draw attention. Northwest A&F University and University of Chinese Academy of Sciences are the key research institutions, and Shen Qirong, Yang Dianlin and Mao Zhiquan are the core author groups, which have made outstanding contributions to the development of this field. The research hotspots have evolved over time, and can be divided into four modules: First, indirect indicators of soil microorganisms (soil enzyme activity, soil uptake, soil microbial biomass carbon and nitrogen); second, mature development of research methods (Phospholipid-derived Fatty Acids, denaturing gradient Gel electrophoresis, Biolog-ECO technique, and high throughput sequencing); third, the visual representation of soil microorganisms (microbial community structure and functional diversity); Lastly, the impact of factors (soil type and land use, fertilization, crops, nitrogen deposition, and bio-char). The application of molecular biological methods such as high throughput sequencing and system biological methods, the development and utilization of bio-char, the influence of nitrogen deposition and the improvement measures should be paid more attention as the focus of future research.

Key words: soil microorganism, CiteSpace, research hotspots, frontier trends

中图分类号:

G353.1

赵丽丽, 张新民. 中国土壤微生物领域研究现状与前沿热点分析[J]. 农业图书情报学报, 2022, 34(2): 75-87.

ZHAO Lili, ZHANG Xinmin. Analysis of Research Status and Frontier Hotspots in Soil Microorganism Field[J]. Journal of Library and Information Science in Agriculture, 2022, 34(2): 75-87.

参考文献

[1] FIERER N, WOOD S A, BUENO DE M C P. How microbes can, and cannot, be used to assess soil health[J]. Soil biology andbiochemistry, 2021: 153.
[2] 宋长青, 吴金水, 陆雅海, 等. 中国土壤微生物学研究 10 年回顾[J]. 地球科学进展, 2013, 28(10): 1087-1105.
SONG C Q, WU J S, LUY H, et al.Advances of soil microbiology in the last decade in China[J]. Advances in earth science, 2013, 28(10): 1087-1105.
[3] 杨良斌. 数据挖掘领域研究现状与趋势的可视化分析[J]. 图书情报工作, 2015, 59(s2): 142-147.
YANG L B.Visual analysis of current situation and trends in the field of data mining[J]. Library and information service, 2015, 59(s2):142-147.
[4] 陈悦, 陈超美, 刘则渊, 等. CiteSpace知识图谱的方法论功能[J]. 科学学研究, 2015, 33(2): 242-253.
CHEN Y, CHEN C M, LIU Z Y, et al.The methodology function of CiteSpace mapping knowledge domains[J]. Studies in science of science, 2015, 33(2): 242-253.
[5] 吴永红, 靳少非. 基于CiteSpace的重金属污染土壤修复研究文献计量分析[J]. 农业环境科学学报, 2020, 39(3): 454-461.
WU Y H, JIN S F.Bibliometric analysis of the repair of heavy met alcontaminated soil based on CiteSpace[J]. Journal of agro-envi-ronment science, 2020, 39(3): 454-461.
[6] 邢素芝, 李孝良, 肖新, 等. 基于CiteSpace可视化分析有机肥料研究进展析[J]. 土壤, 2020, 52(4): 659-667.
XING S Z, LI X L, XIAO X, et al.Visualization analysis of research progress in organic fertilizer study based on CiteSpace[J]. Soils, 2020, 52(4): 659-667.
[7] 孙波, 王晓珗, 吕新华. 我国60年来土壤养分循环微生物机制的研究历程——基于文献计量学和大数据可视化分析[J]. 植物营养与肥料学报, 2017, 23(6): 1590-1601.
SUN B, WANG X Y, LV X H.The historical venation in research on microbial mechanisms of soil nutrient cycling in the past 60 years-Based on bibliometric analysis and big data visualization[J]. Journal of plant nutrition and fertilizer, 2017, 23(6): 1590-1601.
[8] 李杰, 陈超美. 科技文本挖掘及可视化[M]. 北京: 首都经济贸易大学出版社, 2017.
LI J, CHEN C M.CiteSpace: Text mining and scientific literature[M]. Beijing: Capital university of economics and business press, 2017.
[9] 叶鹰. 情报学基础教程[M]. 北京: 科学出版社, 2018.
YE Y.Basic course in intelligence science[M]. Beijing: Science press, 2018.
[10] 曹梦, 李勇, 勾宇轩, 等. 基于知识图谱的土壤中抗生素研究进展分析[J]. 农业资源与环境学报, 2020, 37(5): 627-635.
CAO M, LI Y, GOU Y X, et al.Knowledge mapping analysis of antibiotics in soil research based on the CiteSpace software[J]. Journal of agricultural resources and environment, 2020, 37(5): 627-635.
[11] 杨宁, 邹冬生, 杨满元, 等. 衡阳紫色土丘陵坡地不同植被恢复阶段土壤微生物群落多样性的变化[J]. 林业科学, 2016, 52(8): 146-156.
YANG N, ZOU D S, YANG M Y, et al.Variations of soil microbial community diversity in purple soils at different re-vegetation stages on sloping-land in Hengyang, Hunan province[J]. Scientia silvae sinicae, 2016, 52(8): 146-156.
[12] 王义坤, 孙琪然, 段亚楠, 等. 三种菌肥对苹果连作土壤环境及平邑甜茶幼苗生长的影响[J]. 植物营养与肥料学报, 2019, 25(4): 630-638.
WANG Y K, SUN Q R, DUAN Y N, et al.Effect of three microbial fertilizers on environmental improvement of apple replanted soil and growth of malus hupehensis rehd seedlings[J]. Journal of plantnutrition and fertilizers, 2019, 25(4): 630-638.
[13] 鹿士杨, 彭晚霞, 宋同清, 等. 喀斯特峰丛洼地不同退耕还林还草模式的土壤微生物特性[J]. 生态学报, 2012, 32(8): 2390-2399.
LU S Y, PENG W X, SONG T Q, et al.Soil microbial properties under different grain-for-green patterns in depressions between karsthills[J]. Acta ecologica sinica, 2012, 32(8): 2390-2399.
[14] 赵娜, 林威鹏, 蔡昆争, 等. 家畜粪便堆肥对番茄青枯病、土壤酶活性及土壤微生物功能多样性的影响[J]. 生态学报, 2010, 30(19): 5327-5337.
ZHAO N, LIN W P, CAI K Z, et al.Impact of livestock waste compost on tomato bacterial wilt, soil enzyme activity and soil microbial functional diversity[J]. Acta ecologica sinica, 2010, 30(19): 5327-5337.
[15] 官会林, 郭云周, 张云峰, 等. 绿肥轮作对植烟土壤酶活性与微生物量碳和有机碳的影响[J]. 生态环境学报, 2010, 19(10): 2366-2371.
GUAN H L, GUO Y Z, ZHANG Y F, et al.Effect of green manure rotation soil enzyme activities and soil microbial biomass carbon and soil organic carbon in tobacco field[J]. Ecology and environmental sciences, 2010, 19(10): 2366-2371.
[16] 臧逸飞, 郝明德, 张丽琼, 等. 26年长期施肥对土壤微生物量碳、氮及土壤呼吸的影响[J]. 生态学报, 2015, 35(5): 1445-1451.
ZANG Y F, HAO M D, ZHANG L Q, et al.Effects of wheat cultivation and fertilization on soil microbial biomass carbon, soil microbial biomass nitrogen and soil basal respiration in 26 years[J]. Acta ecologica sinica, 2015, 35(5): 1445-1451.
[17] 涂利华, 戴洪忠, 胡庭兴, 等. 模拟氮沉降对华西雨屏区撑绿杂交竹林土壤呼吸的影响[J]. 应用生态学报, 2011, 22(4): 829-836.
TU L H, DAI H Z, HU T X, et al.Effects of simulated nitrogen deposition on soil respiration in a bambusa pervariabilis dendrocalamopsi plantation in rainy area of west China[J]. Chinese journal of applied ecology, 2011, 22(4): 829-836.
[18] 张奇, 陈粲, 陈效民, 等. 不同深度秸秆还田对黄棕壤氮素和微生物生物量碳氮的影响[J]. 水土保持通报, 2019, 39(2): 56-61.
ZHANG Q, CHEN C, CHEN X M, et al.Effects of straw returning to different soil depth on soil nitrogen and microbial biomass carbon and nitrogen in yellow brown soil[J]. Bulletin of soil and water conservation, 2019, 39(2): 56-61.
[19] 刘素慧, 刘世琦, 张自坤, 等. 大蒜连作对其根际土壤微生物和酶活性的影响[J]. 中国农业科学, 2010, 43(5): 1000-1006.
LIU S H, LIU S Q, ZHANG Z K, et al.Influence of garlic continuous cropping on rhizosphere soil microorganisms and enzyme activities[J]. Scientia agricultura sinica, 2010, 43(5): 1000-1006.
[20] 杨宇虹, 陈冬梅, 晋艳, 等. 不同肥料种类对连作烟草根际土壤微生物功能多样性的影响[J]. 作物学报, 2011, 37(1): 105-111.
YANG Y H, CHEN D M, JIN Y, et al.Effects of different fertilizers on functional diversities of microbial flora in rhizospheric soil of monoculture tobacco[J]. Acta agronomica sinica, 2011, 37(1): 105-111.
[21] 杨滨娟, 黄国勤, 钱海燕. 秸秆还田配施化肥对土壤温度、根际微生物及酶活性的影响[J]. 土壤学报, 2014, 51(1): 150-157.
YANG B J, HUANG G Q, QIAN H Y.Effect of straw incorporation plus chemical fertilizer onsoil temperature, root micro-organisms and enzyme activities[J]. Acta pedologica sinica, 2014, 51(1): 150-157.
[22] 杨广容, 马燕, 蒋宾, 等. 基于16S rDNA测序对茶园土壤细菌群落多样性的研究[J]. 生态学报, 2019, 39(22): 8452-8461.
YANG G R, MA Y, JIANG B, et al.Analysis of the bacterial community and diversity in tea plantation soil via 16s rDNA sequencing[J]. Acta ecologica sinica, 2019, 39(22): 8452-8461.
[23] 王悦, 杨贝贝, 王浩, 等. 不同种植模式下丹参根际土壤微生物群落结构变化[J]. 生态学报, 2019, 39(13): 4832-4843.
WANG Y, YANG B B, WANG H, et al.Variation in microbial community structure in the rhizosphere soil of salvia miltiorrhiza bunge under three cropping modes[J]. Acta ecologica sinica, 2019,39(13): 4832-4843.
[24] 陈正培, 熊建文, 沈方科, 等. Biolog-ECO技术及其应用研究进展[J]. 轻工科技, 2018, 34(6): 97-98, 156.
CHEN Z P, XIONG J W, SHEN F K, et al.Application and research progress of biolog-ECO technology[J]. Light industry science and technology, 2018, 34(6): 97-98, 156.
[25] GE Z W, DU H J, GAO Y L, et al.Analysis on metabolic functions of storedrice microbial communities by biolog ECO microplates[J]. Frontiers in microbiology, 2018, 9: 1375.
[26] 孙雪, 隋心, 韩冬雪, 等. 原始红松林退化演替后土壤微生物功能多样性的变化[J]. 环境科学研究, 2017, 30(6): 911-919.
SUN X, SUI X, HAN D X, et al.Changes of soil microbial functional diversity in the degraded and successional primitive Korean pine forest in lesser Khingan mountain, northern China[J]. Research of environmental sciences, 2017, 30(6): 911-919.
[27] 翟文婷, 陈懂懂, 李奇, 等. 放牧强度对环青海湖地区高寒草原土壤微生物群落碳代谢特征的影响[J]. 应用与环境生物学报, 2017, 23(4): 685-692.
ZHAI W T, CHEN D D, LI Q, et al.Effect of grazing intensity on carbon metabolic characteristics of soil microbial communities in an alpine steppe in the regions around Qinghai lake[J]. Chinese journal of applied and environmental biology, 2017, 23(4): 685-692.
[28] WANG J Q, SHI X Z, ZHENG C Y, et al.Different responses of soil bacterial and fungal communities to nitrogen deposition in a subtropical forest[J]. Science of thetotal environment, 2021, 755: 1.
[29] 赵超, 彭赛, 阮宏华, 等. 氮沉降对土壤微生物影响的研究进展[J]. 南京林业大学学报: 自然科学版, 2015, 39(3): 149-155.
ZHAO C, PENG S, RUAN H H, et al.Effects of nitrogen deposition on soil microbes[J]. Journal of Nanjing forestry university (natural sciences edition), 2015, 39(3): 149-155.
[31] 邓玉峰, 田善义, 成艳红, 等. 模拟氮沉降下施石灰对休耕红壤优势植物根际土壤微生物群落的影响[J]. 土壤学报, 2019, 56(6):1449-1458.
DENG Y F, TIAN S Y, CHENG Y H, et al.Effects of liming on rhizosphere soil microbial communities of dominant plants in fallowed red soil under simulated nitrogen deposition[J]. Acta pedologica sinica, 2019, 56(6): 1449-1458.
[32] ZHU L X, XIAO Q, SHEN Y F, et al.Microbial functional diversity responses to 2 years since biochar application in silt-loam soils on the loess plateau[J]. Ecotoxicology and environmental safety, 2017,144: 578-584.
[33] 王彩云, 武春成, 曹霞, 等. 生物炭对温室黄瓜不同连作年限土壤养分和微生物群落多样性的影响[J]. 应用生态学报, 2019, 30(4): 1359-1366.
WANG C Y, WU C C, CAO X, et al.Effects of biochar on soil nutrition and microbial community diversity under continuous cultivated cucumber soils in greenhouse[J]. Chinese journal of applied ecology, 2019, 30(4): 1359-1366.