利用HRM功能标记检测黑龙江省水稻种质资源Wx和fgr的基因型分布

doi:10.13998/j.cnki.issn1002-1248.2016.10.004

农业图书情报学刊 ›› 2016, Vol. 28 ›› Issue (10): 21-25.doi: 10.13998/j.cnki.issn1002-1248.2016.10.004

利用HRM功能标记检测黑龙江省水稻种质资源Wx和fgr的基因型分布

郭震华^1,2, 罗文龙², 黄翠红², 陈志强², 黄晓群¹, 刘传雪¹, 张兰民¹

1.黑龙江省农业科学院佳木斯水稻研究所,黑龙江佳木斯 154026;
2.国家植物航天育种工程技术研究中心,广东广州 510642

收稿日期:2016-03-22 出版日期:2016-10-05 发布日期:2016-09-29
作者简介:郭震华（1985-）,男,硕士,助理研究员,研究方向：水稻分子育种研究。
基金资助:
黑龙江省农业科技创新工程重点项目（2012ZD021）; 国家水稻产业体系资助项目（CARS-01-14）

Detection on Rice Wx and fgr Gene Distribution in Heilongjiang Rice Germplasm Resource by HRM Functional Markers

GUO Zhen-hua^1,2, LUO Wen-long², HUANG Cui-hong², CHEN Zhi-qiang², HUANG Xiao-qun¹, LIU Chuan-xue¹, ZHANG Lan-min¹

1. Jiamusi Rice Research Institute of Heilongjiang Academy of Agricultural Sciences, Jiamusi 154026;
2. National Engineering Research Centre of Plant Space Breeding, Guangzhou 510642, China

Received:2016-03-22 Online:2016-10-05 Published:2016-09-29

摘要/Abstract

摘要： 水稻直链淀粉含量与香味作为水稻重要的品质性状,一直是水稻研究热点。HRM（高分辨率熔解曲线）技术作为近年来新兴的PCR分析技术,拥有简便、快速及分辨率高等特点,备受关注。为明确黑龙江省种质资源中Wx及fgr基因的分布情况,利用HRM功能标记Wx-a/b和fgr-E7FNP,在67份部分黑龙江省主栽品种及优异种质资源中进行基因分型,结果表明,67份种质均含有纯合Wx^b基因;4份种质含有纯合的fgr基因,分别为哈99352、龙粳香1号、中龙香粳1号及龙交103970,其余63份种质含纯合野生型BAD2基因。

关键词: 水稻, 高分辨率熔解曲线, Wx基因, fgr基因, 基因分型

Abstract: As the most important quality characters of rice grain, amylose content and fragrance are always hot issues in scientific research on rice. HRM (High Resolution Melting) technology as a new PCR analysis technology in recent years, with the characters of simple, fast and high resolution, is widely concerned by the broad mass of researchers. In order to determine the distribution of Wx and fgr gene in germplasm resource of Heilongjiang province, 67 main cultivated varieties and germplasm resource in Heilongjiang province were genotyped by the two HRM(high resolution melting)-based functional markers Wx-a/b and fgr-E7FNP. The results showed that the homozygous gene Wx^b existed in all the 67 germplasm resource; 4 germplasm resource contained the homozygous gene fgr, namely Ha99352, Longjingxiang1, Zhonglongxiangjing1 and Longjiao103970, and the rest contained the homozygous gene BAD2.

Key words: Rice

中图分类号:

S331

郭震华, 罗文龙, 黄翠红, 陈志强, 黄晓群, 刘传雪, 张兰民. 利用HRM功能标记检测黑龙江省水稻种质资源Wx和fgr的基因型分布[J]. 农业图书情报学刊, 2016, 28(10): 21-25.

GUO Zhen-hua, LUO Wen-long, HUANG Cui-hong, CHEN Zhi-qiang, HUANG Xiao-qun, LIU Chuan-xue, ZHANG Lan-min. Detection on Rice Wx and fgr Gene Distribution in Heilongjiang Rice Germplasm Resource by HRM Functional Markers[J]. , 2016, 28(10): 21-25.

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利用HRM功能标记检测黑龙江省水稻种质资源Wx和fgr的基因型分布

Detection on Rice Wx and fgr Gene Distribution in Heilongjiang Rice Germplasm Resource by HRM Functional Markers

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