Abstract: In order to promote the country's construction plan of world-class universities and first-class disciplines, build a powerful country of higher education, this paper analyzed the research trend of constructing the first-class biological disciplines in Southwest University based on Web of Science, InCites and ESI databases by using bibliometric method, and put forward the suggestions for discipline construction. The results showed that the number of biological papers of Southwest University collected in WoS increased year by year but with few scale and poor quality; the number of people who published papers and per capita papers were fewer; the overall quality of international cooperated papers was high but with a low proportion; the core authors were XIA Qingyou and YUAN Ruo and the core colleges were College of Biotechnology, College of Life Science, College of Plant Protection and College of Chemistry and Chemical Engineering; some papers published in publications were not reasonable. Based on above, some related suggestions were put forward: establishing the incentive mechanism of biologic marginal discipline; paying attention to the training and introduction of talents; encouraging and advocating international cooperation; selecting the appropriate journal to publish paper.